; Häder, D.P. Ionizing radiations can affect its expression, reflecting alterations of cell homeostasis [. In this regard, we studied the protective capacity of EDA against the deleterious e ects of 20, no. Deschampsia antarctica je jeden z pouhých dvou druhů krytosemenných rostlin, jež se vyskytují v antarktické oblasti. Ultraviolet A radiation is the predominant component of sunlight causative of photoaging, while ultraviolet B light is considered a potentiator of photoaging. Samples were collected from Antarctica and maintained in vitro culture during two years. Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40–60°C, during 4–6 hours. For that, cells 24 h after the treatments, were incubated with MTT (50 μg/mL) for 3 h. The formazan crystals were dissolved with DMSO and the optical density was determined in a SpectraFluor plate reader (Tecan Trading AG, Switzerland) at a wavelength of 542 nm. Human dermal fibroblast and keratinocytes were seeded on glass coverslips (Menzel-Gläser, Braunschweig, Germany) and when they reached an appropriate confluence (approximately 80%), they were incubated with EDA (0.5 mg/mL) for 24 h. Cells were treated with TDCC or washed with PBS for irradiation with UV light. Exposure of all tested cell types to EDA blunted hallmarks of UVR-induced canonical DNA damage responses and downstream stress/proapoptotic signalling, such as autophagy, caspase activation and MMP1 secretion. and M.M. Kawai, C.; Araújo-Chaves, J.C.; Magrini, T.; Sanches, C.O. Liu, L.; Wise, D.R. Their activity is tightly regulated by endogenous inhibitors the tissue inhibitor of metalloproteinases (TIMPs). Distinct Role of Sesn2 in Response to UVB-Induced DNA Damage and UVA-Induced Oxidative Stress in Melanocytes. Deschampsia antartica (DA) is a polyextremophile plant native to Antarctica . 2019; 20(6):1356. is one of two vascular plants that live in the Maritime Antarctic Territory and is exposed to high levels of UVB. There are no exposure data on these product types. The methods for obtaining the extract have been previously published . Department of Biology, Faculty of Sciences, Autónoma University of Madrid, IRYCIS, 28049 Madrid, Spain, Animal Health Research Center (CISA-INIA), Valdeolmos, 28130 Madrid, Spain, Department of Basic Health Sciences, Faculty of Health Sciences, Rey Juan Carlos University, Alcorcón, 28922 Madrid, Spain, Department of Histology and Pathology, University of Málaga, 29071 Málaga, Spain, Department of Medicine and Medical Specialties, Alcalá de Henares University, 28805 Madrid, Spain. Subsequently, the proteins were transferred to PVDF (Vinylidene Polyfluoride) membranes (Bio-Rad) with the TransBlot Turbo transfer system (Bio-Rad). Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40–60 °C, during 4–6 h. The extract was filtered through a 1μm filter and … This plant lives under high salinity, solar irradiation and oxygen concentration, low temperature and extreme dryness [12]. 2019, 20, 1356. ; Pinto, S.M. Modulation of proteostasis by transcription factor NRF2 and impact in neurodegenerative diseases. ; Budanov, A.; He, Y.Y. In. This research was funded by Industrial Farmacéutica Cantabria and by the Spanish grant from Instituto de Salud Carlos III MINECO and Feder Funds (FIS PI18/00708). A prominent example is embodied by dioxins, highly toxic, persistent organic pollutants of which 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a prototypical species. Rigel, D.S. Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40-60[degrees]C, during 4 … Antioxidant Responses Induced by UVB Radiation in Deschampsia antarctica Desv. Sci. Therefore, the search for opportunities conferring resistance against UV damage and environmental damaging pollutants, is an active field of research. Rothhammer, V.; Quintana, F.J. Exposure of HDF or HaCaT cells to EDA alone for 24 h did not impact visibly on cell culture morphology and confluency (, Then, we set ourselves to test whether UV radiations alter cell homeostasis in our system, and whether EDA modulated these effects. and S.G. Both percentages of positive cells and mean fluorescent intensity for specific antibodies were determined by using the Image J analysis software. The membranes were blocked with skim milk powder or 5% BSA in TBS (Tris-Buffered Saline) 0.1% Tween. Herceg, Z.; Wang, Z.Q. Please note that many of the page functionalities won't work as expected without javascript enabled. Deschampsia antarctica extract (EDAFENCE® hereinafter called EDA) was obtained from IFC, Madrid, Spain. The RCSB PDB also provides a variety of tools and resources. Near confluent HDF monolayers were trypsinized 24 and 48 h after treatment (EDA and/or UVB/A) and processed using a DNAprep kit (Qiagen, Hilden, Germany) according to manufacturer’s recommendations. Semantic Scholar uses AI to extract papers important to this topic. Oxid Med Cell Longev. The present invention relates to natural extracts as a source of therapeutic compounds for human use, specifically for curing and preventing cancerous and tumorous conditions. ; Jung, J.U. A primer on caspase mechanisms. Zamarrón, Alicia; Morel, Esther; Lucena, Silvia R.; Mataix, Manuel; Pérez-Davó, Azahara; Parrado, Concepción; González, Salvador. ; Salvesen, G.S. Santiago-Josefat, B.; Pozo-Guisado, E.; Mulero-Navarro, S.; Fernandez-Salguero, P.M. Proteasome inhibition induces nuclear translocation and transcriptional activation of the dioxin receptor in mouse embryo primary fibroblasts in the absence of xenobiotics. Active components of the antineoplastic extract are also disclosed and a method to prevent proliferation of cancerous or tumorous cells with the extract or with components thereof is disclosed. Cutaneous ultraviolet exposure and its relationship to the development of skin cancer. The role of photochemical quenching and antioxidants in photoprotection of Deschampsia antarctica. To assess whether EDA was specifically impacting on the severity of UVR-induced DNA damage, we recapitulated the treatment conditions outlined above and processed cell cultures for immunofluorescent staining with an antibody recognizing a hallmark of loss of DNA integrity, γH2A.X, for their analysis by fluorescence microscopy [, Furthermore, we assessed a signalling node immediately downstream severe DNA damage and γH2A.X accumulation: polyADP-ribose polymerase (PARP) cleavage [, To better evaluate the functional implications of these observations, we determined whether the attenuation exerted by EDA on UV-induced DNA damage was reflected on differential patterns of proapoptotic signalling. Deschampsia antarctica Desv. The statements, opinions and data contained in the journals are solely While EDA could boost degradation mechanisms targeting TCDD independently of AhR, it is tempting to speculate that EDA exerts direct regulation on AhR, possibly through proteostatic mechanisms such as Hsp90, given its anti-inflammatory activity in vitro [, Generic mechanisms such as antioxidant activity from phenolic compounds in EDA could partly explain a blockade of DNA damage and downstream responses [, Interestingly, our observations support that while UV-induced apparent DNA damage is significantly diminished by simultaneous exposure to EDA, checkpoint mechanisms are still active and cells undergo blockade of their progression through the cell cycle when exposed to UVB light regardless of the presence or absence of EDA. Kudo, I.; Hosaka, M.; Haga, A.; Tsuji, N.; Nagata, Y.; Okada, H.; Fukuda, K.; Kakizaki, Y.; Okamoto, T.; Grave, E.; et al. Functions of poly(ADP-ribose) polymerase (PARP) in DNA repair, genomic integrity and cell death. 2017;2017:2694945. In this work, antioxidant physiology of D. antarctica was studied in response to UVB induced oxidative changes. Deschampsia antarctica and Colobanthus quitensis are the only vascular plants to have colonized the Maritime Antarctic, which is characterized by its permanently low temperature and frequent summer frosts. MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Day, T.A. and M.M. Recommendation for deschampsia antarctica leaf extract flavor usage levels up to: not for flavor use. Author to whom correspondence should be addressed. US20110177178A1 US12/928,317 US92831710A US2011177178A1 US 20110177178 A1 US20110177178 A1 US 20110177178A1 US 92831710 A US92831710 A US 92831710A US 2011177178 A1 US2011177178 A1 US 2011177178A1 Authority US United States Prior art keywords extract compounds extracts deschampsia peaks Prior art date 2007-11-14 Legal status (The legal … You seem to have javascript disabled. Recommendation for deschampsia antarctica leaf extract usage levels up to: not for fragrance use. Pérez-Torres, E.; García, A.; Dinamarca, J.; Alberdi, M.; Gutiérrez, A.; Gidekel, M.; Ivanov, A.G.; Hüner, N.P. ; Xiong, F.S. ; Diehl, J.A. High throughput sequencing showed that up to 92% of DNA reads, and 68% of RNA reads, in soils from the islands were accounted for by the fungus. These protective effects were independent from modulation of cell cycle progression. those of the individual authors and contributors and not of the publisher and the editor(s). Subscribe to receive issue release notifications and newsletters from MDPI journals, You can make submissions to other journals. Deschampsia antartica (DA) is a polyextremophile plant native to Antarctica [11]. Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40–60 °C, during 4–6 h. The extract was filtered through a 1μm filter and lyophilized. Pajares, M.; Cuadrado, A.; Rojo, A.I. Find support for a specific problem on the support section of our website. therefore being studied. An Extract from the Plant Deschampsia antarctica Protects Fibroblasts from Senescence Induced by Hydrogen Peroxide Oxidative Medicine and Cellular Longevity , Aug 2017 Ana Ortiz-Espín , Esther Morel , Ángeles Juarranz , Antonio Guerrero , Salvador González , Ana Jiménez , Francisca Sevilla Quan, T.; Qin, Z.; Xia, W.; Shao, Y.; Voorhees, J.J.; Fisher, G.J. Ortiz-Espín, A.; Morel, E.; Juarranz, Á.; Guerrero, A.; González, S.; Jiménez, A.; Sevilla, F. An Extract from the Plant Deschampsia antarctica Protects Fibroblasts from Senescence Induced by Hydrogen Peroxide. In this work, antioxidant physiology of D. antarctica was studied in response to UVB induced oxidative changes. In addition, different chemicals contribute to skin aging upon penetration through skin barrier disruption or hair follicles, aryl hydrocarbon receptors (AhR) being a major effector mechanism through which toxicity is exerted. An extract from the plant Deschampsia antarctica protects fibroblasts from senescence induced by hydrogen peroxide. The regulation mechanisms of AhR by molecular chaperone complex. 2019. Photodamage in a mitochondrial membrane model modulated by the topology of cationic and anionic meso-tetrakis porphyrin free bases. those of the individual authors and contributors and not of the publisher and the editor(s). These authors contributed equally to this work. We also thank Carmen Moreno-Ortiz from Flow Cytometry, Centro Nacional de Biotecnología (Madrid, Spain). Multiple requests from the same IP address are counted as one view. Fragrance ingredients in hair permanent and other hair chemical treatments have been placed in Category 7. This plant lives under high salinity, solar irradiation and oxygen concentration, low temperature and extreme dryness . Bennett, B.T. In addition, different chemicals contribute to skin aging upon penetration through skin barrier disruption or hair follicles, aryl hydrocarbon receptors (AhR) being a major effector mechanism through which toxicity is exerted. Zamarrón, A.; Morel, E.; Lucena, S.R. in vitro studies have shown that DA aqueous extract (DAE) can down-regulate the pollutant-induced AhR activation . These results might encourage future mechanistic studies as well as assessment in in vivo models. In order to study the potential protective properties of EDA against UV radiation, we first assessed whether EDA alters cell physiology on its own—in model skin cell cultures we studied in parallel human dermal fibroblasts (hereon HDF cells) and an established human keratinocyte cell line (HaCaT). Poly ( ADP-ribose ) polymerase ( PARP ) in DNA repair, genomic integrity and cell survival,. Barcelona, Spain therefore, the search for opportunities conferring resistance against UV damage and damaging! And 15.9 W/m it susceptible of developing different pathologies, including cancer and 15.9 W/m exposure and relationship... Ultraviolet a radiation is the predominant component of sunlight causative of photoaging μg of total or... Thank Carmen Moreno-Ortiz from Flow Cytometry, Centro Nacional de Biotecnología (,. Were independent from modulation of proteostasis by transcription factor NRF2 and impact in diseases. 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Were collected from antarctica and maintained in DMEM in the journal, 1996-2020. Healthy differentiation of keratinocytes pollutants of which 2,3,7,8-Tetrachlorodibenzo-p-dioxin ( TCDD ) is a prototypical species, cell culture medium replaced! Users who range from students to specialized scientists skin cancer pigmentation different from students to specialized scientists learn about. ) exerts deschampsia antarctica extract protective effects on human skin cell against dioxins treatments ; Cuadrado, A. ;,!: an environmental sensor integrating immune responses in health and disease the pollutant-induced AhR.! Modulation of cell cycle progression model modulated by the topology of cationic and anionic meso-tetrakis porphyrin bases... There are no exposure data on these product types specific problem on the support section our... And impact in neurodegenerative diseases in all cases, downloaded, and analyzed by users who range students. 300–400 nm and 15.9 W/m damage response proteins: Optimizing protocols for super-resolution microscopy solar irradiation and oxygen concentration low! To other journals Martinho, H. ; Daghastanli, N. ; Nantes I.L... Is exposed to high levels of UVB Qin, Z. ; Xia, W. Shao! Viability in HaCaT and HDF cells after different treatments was determined by MTT assay search for opportunities conferring against... Ahr activation [ 13 ] is exposed to high levels of UVB Rojo, A.I of or! Intensity for specific antibodies were determined by MTT assay and alterations in different!